The Roche/454 GS FLX System is based on emulsion PCR [8] and pyrophosphate detection [9] techniques. A library of DNA templates is constructed by a highly efficient DNA amplification method known as ...
DNA methylation is a form of epigenetics in which gene expression is encouraged or inhibited by the addition of a methyl group on cytosines. It is highly implicated in development, cell ...
Investigation of archaeal and bacterial diversity in fermented seafood using barcoded pyrosequencing
The seven kinds of seafood samples were purchased from the distributors of commercially available brands in Korea. These foods were made of the following raw materials: tiny shrimp (designated J1), ...
Multiple fields of microbiology rely on techniques of microbial identification, such as in food microbiology for detecting contaminants, and in microbial ecology to characterize biodiversity. Image ...
A technique that combines the speed of pyrosequencing with the sensitivity of fluorescent detection may lead to faster sequencing with smaller quantities of DNA. The method described by Sims et al.
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